nicotinamide→NAD→Macromolecular Substances→Protein-Serine-Threonine Kinases→poly-ADP-ribose

• 10509758(nicotinamide): Murine pharmacokinetics of 6-aminonicotinamide (NSC 21206), a novel biochemical modulating agent.

  K562 cells displayed progressive increases in Pt-DNA adducts and cisplatin sensitivity during the first 10 hr of 6AN exposure.

• 9516960(NAD): 6-Aminonicotinamide sensitizes human tumor cell lines to cisplatin.

  Morphological examination revealed increased numbers of apoptotic cells after treatment with 6AN and cisplatin compared to cisplatin alone.

• 9377558(Macromolecular Substances): Induction of apoptosis in cisplatin-sensitive and -resistant human ovarian cancer cell lines.

  Forty-eight h following cisplatin treatment , unattached , apoptotic A2780 cells demonstrated a 20-30 % decrease in DNA-PK phosphorylation activity.

• 9036952(Protein-Serine-Threonine Kinases): The DNA-dependent protein kinase catalytic subunit (p460) is cleaved during Fas-mediated apoptosis in Jurkat cells.

  The DNA-dependent protein kinase (DNA-PK) is a serine/threonine kinase linked to DNA repair and V (D) J recombination.

• 6309564(poly-ADP-ribose): ADP-ribosylation regulates the phosphorylation of histones by the catalytic subunit of cyclic AMP-dependent protein kinase.

  Phosphorylation of whole histones from calf thymus by the catalytic subunit of cyclic AMP-dependent protein kinase was markedly reduced when the histones were ADP-ribosylated.