glutamine→polyglutamine→glutamine-synthetase adenylyltransferase→Adenosine Monophosphate→pyruvate kinase

• 16469881(glutamine): Progressive disruption of cellular protein folding in models of polyglutamine diseases.

  The expansion of polyglutamine residues in unrelated proteins is associated with the early onset of neurodegenerative disease.

• 8980137(polyglutamine): Toxicity of expanded polyglutamine-domain proteins in Escherichia coli.

  In this paper , we demonstrate in E. coli that long polyglutamine domains (59-81 residues) as GST-fusion proteins inhibit growth while smaller glutamine (10-35 residues) or polyalanine (61 residues) domains have no effect.

• 16511122(glutamine-synthetase adenylyltransferase): Expression, purification and crystallization of the C-terminal domain of Escherichia coli adenylyltransferase.

  The domain is contained in a fragment that extends from residues 441-945 of the intact ATase.

• 14766310(Adenosine Monophosphate): Expression, purification, crystallization, and preliminary X-ray analysis of the N-terminal domain of Escherichia coli adenylyl transferase.

  A soluble N-terminal domain of the Escherichia coli adenylyl transferase (ATase) is responsible for deadenylylation activity of the intact enzyme.

• 16511150(pyruvate kinase): Crystallization and preliminary X-ray analysis of pyruvate kinase from Bacillus stearothermophilus.

  A crystal suitable for structural analysis was obtained using C9S/C268S enzyme.