pyruvate kinase→Riboflavin→Pyruvate Kinase→glutamine

• 17550844(pyruvate kinase): Transgenic rescue of hemolytic anemia due to red blood cell pyruvate kinase deficiency.

  Red blood cell pyruvate kinase (R-PK) deficiency is the most common glycolytic enzyme defect associated with hereditary non-spherocytic hemolytic anemia.

• 963898(Riboflavin): Effect of riboflavin treatment on human red cell pyruvate kinase deficiency.

  The effect of riboflavin on six patients with hemolytic anemia due to hereditary pyruvate kinase deficiency was studied.

• 1159538(Pyruvate Kinase): Increased activity of pyruvate kinase in plasma of vitamin E-deficient rats.

  The results suggest that vitamine E deficiency in rats may cause muscular damage and release of pyruvate kinase into blood circulation.

• 8748364(glutamine): Effect of glutamine supplementation on protein metabolism and glutathione in tumor-bearing rats.

  Since tumor-bearing rats are deficient in glutamine , we investigated whether (1) glutamine and glutathione deficiency occur in tumor-bearing rats , (2) glutamine supplementation caused an increase of glutathione levels in host tissues and tumor , (3) glutamine enhances protein synthesis in host tissues , and (4) glutamine stimulated the tumor to synthesize protein and DNA.